diff --git a/_pytest/fixtures.py b/_pytest/fixtures.py
index 64d21b9f6..b9d1070ce 100644
--- a/_pytest/fixtures.py
+++ b/_pytest/fixtures.py
@@ -827,6 +827,10 @@ class FixtureFunctionMarker:
         if isclass(function):
             raise ValueError(
                     "class fixtures not supported (may be in the future)")
+        if getattr(function, "_pytestfixturefunction", False):
+            raise ValueError(
+                    "fixture is being applied more than once to the same function")
+
         function._pytestfixturefunction = self
         return function
 
diff --git a/changelog/2334.feature b/changelog/2334.feature
new file mode 100644
index 000000000..5aec67994
--- /dev/null
+++ b/changelog/2334.feature
@@ -0,0 +1 @@
+Now when @pytest.fixture is being applied to the same function ValueError is raised.
\ No newline at end of file