diff --git a/changelog/12564.bugfix.rst b/changelog/12564.bugfix.rst
new file mode 100644
index 000000000..344bf8b7e
--- /dev/null
+++ b/changelog/12564.bugfix.rst
@@ -0,0 +1 @@
+Fix the ``stacklevel`` used when warning about marks used on fixtures.
diff --git a/src/_pytest/fixtures.py b/src/_pytest/fixtures.py
index 0151a4d9c..1f0740d09 100644
--- a/src/_pytest/fixtures.py
+++ b/src/_pytest/fixtures.py
@@ -1192,7 +1192,7 @@ class FixtureFunctionMarker:
             )
 
         if hasattr(function, "pytestmark"):
-            warnings.warn(MARKED_FIXTURE, stacklevel=2)
+            warnings.warn(MARKED_FIXTURE, stacklevel=4)
 
         function = wrap_function_to_error_out_if_called_directly(function, self)
 
diff --git a/testing/deprecated_test.py b/testing/deprecated_test.py
index 5d0e69c58..0fd2e9510 100644
--- a/testing/deprecated_test.py
+++ b/testing/deprecated_test.py
@@ -185,6 +185,8 @@ def test_fixture_disallow_on_marked_functions():
     # from applying @fixture twice
     # ValueError("fixture is being applied more than once to the same function")
     assert len(record) == 1
+    # should point to this file
+    assert record[0].filename == __file__
 
 
 def test_fixture_disallow_marks_on_fixtures():