diff --git a/AUTHORS b/AUTHORS
index ad7fccf6d..26fa27f9b 100644
--- a/AUTHORS
+++ b/AUTHORS
@@ -454,6 +454,7 @@ Yusuke Kadowaki
 Yutian Li
 Yuval Shimon
 Zac Hatfield-Dodds
+Zach Snicker
 Zachary Kneupper
 Zachary OBrien
 Zhouxin Qiu
diff --git a/src/_pytest/main.py b/src/_pytest/main.py
index 47ebad471..0b9e64e2f 100644
--- a/src/_pytest/main.py
+++ b/src/_pytest/main.py
@@ -369,22 +369,11 @@ def pytest_runtestloop(session: Session) -> bool:
 
 def _in_venv(path: Path) -> bool:
     """Attempt to detect if ``path`` is the root of a Virtual Environment by
-    checking for the existence of the appropriate activate script."""
-    bindir = path.joinpath("Scripts" if sys.platform.startswith("win") else "bin")
+    checking for the existence of the pyvenv.cfg file."""
     try:
-        if not bindir.is_dir():
-            return False
+        return path.joinpath("pyvenv.cfg").exists()
     except OSError:
         return False
-    activates = (
-        "activate",
-        "activate.csh",
-        "activate.fish",
-        "Activate",
-        "Activate.bat",
-        "Activate.ps1",
-    )
-    return any(fname.name in activates for fname in bindir.iterdir())
 
 
 def pytest_ignore_collect(collection_path: Path, config: Config) -> bool | None:
diff --git a/testing/test_collection.py b/testing/test_collection.py
index 821c42419..f58222403 100644
--- a/testing/test_collection.py
+++ b/testing/test_collection.py
@@ -152,20 +152,8 @@ class TestCollectFS:
         assert "test_notfound" not in s
         assert "test_found" in s
 
-    @pytest.mark.parametrize(
-        "fname",
-        (
-            "activate",
-            "activate.csh",
-            "activate.fish",
-            "Activate",
-            "Activate.bat",
-            "Activate.ps1",
-        ),
-    )
-    def test_ignored_virtualenvs(self, pytester: Pytester, fname: str) -> None:
-        bindir = "Scripts" if sys.platform.startswith("win") else "bin"
-        ensure_file(pytester.path / "virtual" / bindir / fname)
+    def test_ignored_virtualenvs(self, pytester: Pytester) -> None:
+        ensure_file(pytester.path / "virtual" / "pyvenv.cfg")
         testfile = ensure_file(pytester.path / "virtual" / "test_invenv.py")
         testfile.write_text("def test_hello(): pass", encoding="utf-8")
 
@@ -179,23 +167,11 @@ class TestCollectFS:
         result = pytester.runpytest("virtual")
         assert "test_invenv" in result.stdout.str()
 
-    @pytest.mark.parametrize(
-        "fname",
-        (
-            "activate",
-            "activate.csh",
-            "activate.fish",
-            "Activate",
-            "Activate.bat",
-            "Activate.ps1",
-        ),
-    )
     def test_ignored_virtualenvs_norecursedirs_precedence(
-        self, pytester: Pytester, fname: str
+        self, pytester: Pytester
     ) -> None:
-        bindir = "Scripts" if sys.platform.startswith("win") else "bin"
         # norecursedirs takes priority
-        ensure_file(pytester.path / ".virtual" / bindir / fname)
+        ensure_file(pytester.path / ".virtual" / "pyvenv.cfg")
         testfile = ensure_file(pytester.path / ".virtual" / "test_invenv.py")
         testfile.write_text("def test_hello(): pass", encoding="utf-8")
         result = pytester.runpytest("--collect-in-virtualenv")
@@ -204,27 +180,13 @@ class TestCollectFS:
         result = pytester.runpytest("--collect-in-virtualenv", ".virtual")
         assert "test_invenv" in result.stdout.str()
 
-    @pytest.mark.parametrize(
-        "fname",
-        (
-            "activate",
-            "activate.csh",
-            "activate.fish",
-            "Activate",
-            "Activate.bat",
-            "Activate.ps1",
-        ),
-    )
-    def test__in_venv(self, pytester: Pytester, fname: str) -> None:
+    def test__in_venv(self, pytester: Pytester) -> None:
         """Directly test the virtual env detection function"""
-        bindir = "Scripts" if sys.platform.startswith("win") else "bin"
-        # no bin/activate, not a virtualenv
+        # no pyvenv.cfg, not a virtualenv
         base_path = pytester.mkdir("venv")
         assert _in_venv(base_path) is False
-        # with bin/activate, totally a virtualenv
-        bin_path = base_path.joinpath(bindir)
-        bin_path.mkdir()
-        bin_path.joinpath(fname).touch()
+        # with pyvenv.cfg, totally a virtualenv
+        base_path.joinpath("pyvenv.cfg").touch()
         assert _in_venv(base_path) is True
 
     def test_custom_norecursedirs(self, pytester: Pytester) -> None: