diff --git a/src/_pytest/doctest.py b/src/_pytest/doctest.py
index d0b6b4c41..24f888257 100644
--- a/src/_pytest/doctest.py
+++ b/src/_pytest/doctest.py
@@ -36,6 +36,7 @@ from _pytest.config.argparsing import Parser
 from _pytest.fixtures import FixtureRequest
 from _pytest.nodes import Collector
 from _pytest.outcomes import OutcomeException
+from _pytest.pathlib import fnmatch_ex
 from _pytest.pathlib import import_path
 from _pytest.python_api import approx
 from _pytest.warning_types import PytestWarning
@@ -120,32 +121,32 @@ def pytest_unconfigure() -> None:
 
 
 def pytest_collect_file(
-    path: py.path.local, parent: Collector,
+    fspath: Path, path: py.path.local, parent: Collector,
 ) -> Optional[Union["DoctestModule", "DoctestTextfile"]]:
     config = parent.config
-    if path.ext == ".py":
-        if config.option.doctestmodules and not _is_setup_py(path):
+    if fspath.suffix == ".py":
+        if config.option.doctestmodules and not _is_setup_py(fspath):
             mod: DoctestModule = DoctestModule.from_parent(parent, fspath=path)
             return mod
-    elif _is_doctest(config, path, parent):
+    elif _is_doctest(config, fspath, parent):
         txt: DoctestTextfile = DoctestTextfile.from_parent(parent, fspath=path)
         return txt
     return None
 
 
-def _is_setup_py(path: py.path.local) -> bool:
-    if path.basename != "setup.py":
+def _is_setup_py(path: Path) -> bool:
+    if path.name != "setup.py":
         return False
-    contents = path.read_binary()
+    contents = path.read_bytes()
     return b"setuptools" in contents or b"distutils" in contents
 
 
-def _is_doctest(config: Config, path: py.path.local, parent) -> bool:
-    if path.ext in (".txt", ".rst") and parent.session.isinitpath(path):
+def _is_doctest(config: Config, path: Path, parent: Collector) -> bool:
+    if path.suffix in (".txt", ".rst") and parent.session.isinitpath(path):
         return True
     globs = config.getoption("doctestglob") or ["test*.txt"]
     for glob in globs:
-        if path.check(fnmatch=glob):
+        if fnmatch_ex(glob, path):
             return True
     return False
 
diff --git a/testing/test_doctest.py b/testing/test_doctest.py
index 6e3880330..08d0aacf6 100644
--- a/testing/test_doctest.py
+++ b/testing/test_doctest.py
@@ -1,10 +1,9 @@
 import inspect
 import textwrap
+from pathlib import Path
 from typing import Callable
 from typing import Optional
 
-import py
-
 import pytest
 from _pytest.doctest import _get_checker
 from _pytest.doctest import _is_mocked
@@ -1496,25 +1495,25 @@ def test_warning_on_unwrap_of_broken_object(
     assert inspect.unwrap.__module__ == "inspect"
 
 
-def test_is_setup_py_not_named_setup_py(tmp_path):
+def test_is_setup_py_not_named_setup_py(tmp_path: Path) -> None:
     not_setup_py = tmp_path.joinpath("not_setup.py")
     not_setup_py.write_text('from setuptools import setup; setup(name="foo")')
-    assert not _is_setup_py(py.path.local(str(not_setup_py)))
+    assert not _is_setup_py(not_setup_py)
 
 
 @pytest.mark.parametrize("mod", ("setuptools", "distutils.core"))
-def test_is_setup_py_is_a_setup_py(tmpdir, mod):
-    setup_py = tmpdir.join("setup.py")
-    setup_py.write(f'from {mod} import setup; setup(name="foo")')
+def test_is_setup_py_is_a_setup_py(tmp_path: Path, mod: str) -> None:
+    setup_py = tmp_path.joinpath("setup.py")
+    setup_py.write_text(f'from {mod} import setup; setup(name="foo")', "utf-8")
     assert _is_setup_py(setup_py)
 
 
 @pytest.mark.parametrize("mod", ("setuptools", "distutils.core"))
-def test_is_setup_py_different_encoding(tmp_path, mod):
+def test_is_setup_py_different_encoding(tmp_path: Path, mod: str) -> None:
     setup_py = tmp_path.joinpath("setup.py")
     contents = (
         "# -*- coding: cp1252 -*-\n"
         'from {} import setup; setup(name="foo", description="€")\n'.format(mod)
     )
     setup_py.write_bytes(contents.encode("cp1252"))
-    assert _is_setup_py(py.path.local(str(setup_py)))
+    assert _is_setup_py(setup_py)